Diffusion of RhoB in P407. All data is depicted as RhoB concentration in the receptor chamber, normalized by membrane area, as a function of time. Results are color-coded by experiment type, as indicated by legend and above schematic. (a) Predicted concentration profiles for 0 holes (solid lines), along with raw data shown (transparent lines). 32 total experiments represented. (b) Diffusion of RhoB in P407. Predicted concentration profiles for holes with same total cross sectional area (one hole 200 μm diameter, four holes 100 μm diameter) in P407 with raw data. 38 total experiments represented.