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. 2019 Aug 26;47(17):8950–8960. doi: 10.1093/nar/gkz729

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Ligation reaction of minF1 and FAM-F1*subA (FAM: 6-fluorescein label). minF1 was in excess and the samples were analyzed by denaturing PAGE. FAM fluorescence was detected by a gel imager. The experiment was repeated three times, and the data were fitted to the equation FL = F_max(1 – e⁻^k_obs^t) which is represented by the solid curve. A representative gel electrophoregram is shown in inset.