Table 1.

Relative activities of F1* mutants in the G33-A35 bulge

Bulge sequence	RA	Bulge sequence	RA	Bulge sequence	RA	Bulge sequence	RA
GAA (WT)	1.00	3-nt bulge, double mutants		3-nt bulge, triple mutants		2-nt bulge
3-nt bulge, single mutants		acA	0.93	acc	0.06	AA	1.07
aAA	1.05	agA	1.05	acg	0.10	AC	0.71
cAA	0.99	auA	1.02	acu	0.19	AG	0.99
uAA	1.06	ccA	0.28	agc	0.17	AU	0.97
GcA	0.98	cgA	1.08	agg	0.43	CA	1.01
GgA	1.00	cuA	0.94	agu	0.19	CC	0.03
GuA	1.03	ucA	0.02	auc	0.02	CG	0.03
GAc	1.04	ugA	1.03	aug	0.18	CU	0.02
GAg	1.05	uuA	0.48	auu	0.21	GA	1.08
GAu	1.08	aAc	1.00	ccc	0.03	GC	1.02
		aAg	1.06	ccg	0.03	GG	0.45
		aAu	1.02	ccu	0.04	GU	0.45
		cAc	0.53	cgc	0.13	UA	1.07
		cAg	0.99	cgg	0.06	UC	0.01
		cAu	0.81	cgu	0.03	UG	0.03
		uAc	0.92	cuc	0.01	UU	0.02
		uAg	1.01	cug	0.04	1-nt bulge
		uAu	1.01	cuu	0.02	A	0.99
		Gcc	0.57	ucc	0.00	C	0.02
		Gcg	0.03	ucg	0.01	G	0.03
		Gcu	0.98	ucu	0.01	U	0.02
		Ggc	0.96	ugc	0.08	No bulge
		Ggg	1.03	ugg	0.05	-	0.03
		Ggu	0.49	ugu	0.03
		Guc	0.10	uuc	0.01
		Gug	0.28	uug	0.01
		Guu	0.58	uuu	0.02

For the 3-nt bulge mutants, the mutations are indicated in lowercase.