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. 2019 Aug 8;47(17):9259–9270. doi: 10.1093/nar/gkz676

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — The CRISPR system of Mycobacterium tuberculosis. (A) The CRISPR locus of M. tuberculosis includes genes encoding Cas6 (crRNA processing), Csm1–5 (type III-A interference complex), Csm6 (ancillary ribonuclease), Cas1 and Cas2 (Adaptation). Cas6 cleaves the CRISPR RNA at the base of a short hairpin to generate mature crRNA that is bound by the Csm complex. On target RNA binding, the Csm complex is expected to display three enzymatic activities: target RNA cleavage by Cas7 (Csm3) (1), DNA cleavage by the HD domain (2) and cOA production by the cyclase domain (3). (B) Purified, recombinant CRISPR-associated proteins of M. tuberculosis. M: PageRuler Unstained (Thermo Scientific); 1: Csm1–5 interference complex; 2: Csm1–5, Csm1 D630A, D631A (Cy variant); 3: Csm1–5, Csm3 D35A (C3 variant); 4: Csm6; 5: Cas6.