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Target RNA cleavage by the Csm complex. WT, Csm3 D35A variant (C3) or cyclase variant (Cy) Csm were incubated with 5′-32P-target RNA under single-turnover conditions for 0.5, 2, 5, 30, 90 min and analysed by denaturing PAGE. Five RNA cleavage sites (B1–B5) were observed.
