Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 8;47(17):9259–9270. doi: 10.1093/nar/gkz676

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 4. — Plasmid immunity assays of Mtb Csm in heterologous host Escherichia coli. (A) C43 harbouring pCsm1–6 and pCRISPR are transformed with pRAT (control plasmid) or with pRAT-target (plasmid with pUC19 MCS target). Target transcription was arabinose-inducible. In the absence of arabinose, the transformation efficiency was independent of the presence or absence of target; whereas when target transcription was induced, a 2-log reduction of transformation efficiency was observed for the target plasmid compared to the control plasmid. *** P-value (Welch two sample test, two tailed) < 1e-05; significance threshold at < 0.05. (B) C43 harbouring pCsm1–6 or indicated mutants and pCRISPR_TetR (target tetracycline resistance gene, constitutively expressed) were transformed with pRAT. Vector control (Ctrl): backbone vectors without inserts; WT Csm, Csm1–5 only (ΔCsm6, C6), Csm3 D35A (C3), Csm1 (Cas10) D630A/D631A (cyclase mutant, Cy), Cas10 H18A/D19A (HD mutant, HD).