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. 2019 Aug 10;47(17):9448–9463. doi: 10.1093/nar/gkz703

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Bp OLD exhibits metal-dependent nuclease activity. (A) Domain architecture of Bp OLD. Boundaries of the CTR construct is labeled. (B, C). Metal-dependent nuclease activity of Bp^FL (B) and Bp^CTR (C) on linear λ DNA. ‘λ’ denotes the position of uncut linear DNA. (D, E) Metal-dependent nuclease activity of Bp^FL (D) and Bp^CTR (E) on supercoiled pUC19 DNA. ‘N’, ‘L’, and ‘S’ denote the positions of ‘nicked’, ‘linearized’, and ‘supercoiled’ DNA respectively. Cartoons of these respective products are illustrated in D next to their label. For B–E, lanes labeled with dashed lines indicate samples with no metal added. DNA degradation was quantified using BioRad Image Lab software as described in the Materials and Methods. Bar graphs represent the average of three independent experiments with error bars representing the standard error of the mean.