Figure 3.

Identification of PaTrmB tRNA substrates. In vitro transcribed tRNAs were used as substrates for the purified PaTrmB, with SAM as methyl donor. Methylation reaction was assessed using an MTase-Glo™ Methyltransferase Assay Kit (Promega) according to the manufacturer's instructions. The reaction without PaTrmB served as control. The tRNA transcripts containing G46 are shown as black bars, whereas those lacking G46 are shown as white bars. Data represent the mean ± SD for three biological replicates.