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. 2019 Aug 20;47(17):9271–9281. doi: 10.1093/nar/gkz702

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — TrmB affects the translation of codons decoded by tRNA^Phe/GAA and tRNA^Asp/GUC. (A) Design of the reporter system. Each synthesized fragment was cloned into the pPR9TT-P_ApR vector in frame with lacZ under the control of the P_ApR constitutive promoter. Relative translation efficiencies of (B) 5XGln-CAA reporter (negative control), (C) 5XPhe-UUC reporter, (D) 5XPhe-UUU reporter, (E) 5XAsp-GAC reporter and (F) 5XAsp-GAU reporter in the wild type and trmB mutant strains after H₂O₂ exposure. Data represent the mean ± SD for four biological replicates. Asterisks denote a significant difference by t-test (**P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001). Q, glutamine; F phenylalanine; D, aspartic acid.