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. 2019 Aug 21;47(17):9343–9357. doi: 10.1093/nar/gkz678

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — DCL-mediated generation of TBSV siRNAs in BYL. (A) Schematic representation of the in vitro ‘Dicer assay’ performed with double-stranded TBSV RNA. (B) Size distribution of sequenced TBSV siRNAs. Bars above the axis represent siRNAs derived from viral (+)RNA, bars below the axis represent siRNAs derived from viral (-)RNA. (C) Distribution and abundance of the sequenced 21 nt vsiRNAs aligned to the TBSV genome. Peaks above the axis represent siRNAs derived from viral (+)RNA, peaks below the axis represent siRNAs derived from viral (-)RNA. The peaks indicate the position of either the 5′ nucleotide of a vsiRNA with respect to the TBSV genome (in case of (+)vsiRNAs) or the TBSV genome position complementary to the 5′ nucleotide of a vsiRNA (in case of (-)vsiRNAs). The locations of the three most abundant (-)vsiRNA reads are specified. The chart on the right represents the relative abundance of the sum of (+) and (-)RNA derived siRNAs, respectively. Data represent mean ± S.E.M.