Table 1.
Most abundant AGO/RISC-associated TBSV (-)siRNAs; datasets categorized as explained in the text and below
| Dataset 1a | |||||
|---|---|---|---|---|---|
| AGO1 | AGO2 | ||||
| vsiRNA | 5′ nt | Mean abundance (%) | vsiRNA | 5′ nt | Mean abundance (%) |
| 3939 | U | 1.59 | 4418 | A | 9.13 |
| 3516 | A | 1.30 | 3758 | A | 4.96 |
| 4123 | U | 1.06 | 3701 | A | 1.72 |
| 864 | U | 0.96 | 3593 | A | 0.92 |
| 3257 | U | 0.95 | 3342 | A | 0.76 |
| 3722 | U | 0.90 | 4771 | A | 0.76 |
| 4643 | U | 0.81 | 4044 | A | 0.70 |
| 1823 | U | 0.80 | 3698 | A | 0.66 |
| 1230 | U | 0.77 | 3243 | A | 0.63 |
| 1164 | U | 0.73 | 1470 | A | 0.53 |
| Dataset 2b | |||||
| AGO1 | AGO2 | ||||
| vsiRNA | 5′ nt | log2 Fold change | vsiRNA | 5′ nt | log2 Fold change |
| 3722 | U | 7.72 | 3701 | A | 8.31 |
| 1575 | U | 7.04 | 4418 | A | 7.55 |
| 2678 | U | 6.77 | 3758 | A | 7.52 |
| 3939 | U | 6.54 | 3698 | A | 6.93 |
| 4336 | U | 6.54 | 3107 | A | 6.49 |
| 4037 | U | 6.11 | 1221 | A | 6.38 |
| 1680 | U | 5.98 | 4415 | A | 6.30 |
| 3927 | U | 5.92 | 186 | A | 6.27 |
| 343 | U | 5.87 | 4771 | A | 6.13 |
| 3257 | U | 5.80 | 3943 | A | 5.86 |
| Dataset 3c | |||||
| AGO1 | AGO2 | ||||
| vsiRNA | 5′ nt | Mean abundance (%) | vsiRNA | 5′ nt | Mean abundance (%) |
| 179 | U | 0.38 | 4044 | A | 0.70 |
| 1717 | U | 0.29 | 3243 | A | 0.63 |
| 4052 | U | 0.26 | 1470 | A | 0.53 |
| 885 | U | 0.26 | 3704 | A | 0.53 |
| 4667 | U | 0.21 | 2710 | A | 0.39 |
| 1324 | U | 0.20 | 3492 | A | 0.34 |
| 1252 | U | 0.19 | 2921 | A | 0.33 |
| 1574 | U | 0.19 | 340 | A | 0.31 |
| 4585 | U | 0.19 | 261 | A | 0.27 |
| 1432 | U | 0.17 | 432 | A | 0.23 |
avsiRNAs were listed exclusively according to their frequency in AGO/RISC immunoprecipitation.
bvsiRNAs were sorted by their accumulation in AGO/RISC immunoprecipitation compared to their frequency in the DCL assay.
cvsiRNAs, not detectable in the DCL assay but readily detectable in the AGO/RISC immunoprecipitation, were listed according to their frequency in the latter.