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. 2019 Sep 4;203(7):1766–1775. doi: 10.4049/jimmunol.1900386

FIGURE 1.

FIGURE 1.

mTNF expression is increased in RA monocytes. (A) Gating strategy used to determined expression of mTNF on monocytes subpopulation on PBMCs. (B and C) Ex vivo monocyte mTNF expression on HD (n = 23), RA (n = 42), SS (n = 18), SLE (n = 6), and SpA (n = 15) patients was determined in flow cytometry analyses after PBMCs isolation by Pancoll separation with anti–HLA-DR, anti-CD2, anti-CD19, anti-CD56, anti-CD14, anti-CD16, and anti-mTNF Ab. (D) Scatterplot showed a significant positive correlation between monocyte mTNF expression (geometric mean fluorescence intensity [gMFI]) and the DAS28-CRP in RA patients (n = 42). (E) Purified blood monocytes treated with TNFi (ETA or ADA) or with IVIg were harvested after 3 d and analyzed for monocytes mTNF expression with anti-CD14, anti-mTNF, and live death (HD n = 6 and RA n = 6). Results are shown as mean ± SEM. Kruskal–Wallis test with Dunn multiple comparisons and the two-tailed nonparametric Spearman for correlation. *p ≤ 0.05, **p ≤ 0.01.