Skip to main content
. 2019 Sep 4;203(7):1766–1775. doi: 10.4049/jimmunol.1900386

FIGURE 6.

FIGURE 6.

M-CSF signaling pathway and miR-155 involvement. (A) The figure represents the interaction between CSF-1 and its receptor CSF-1R, downstream signaling pathway, and involvement of miR-155. (B) Ex vivo monocyte CD115 expression on HD (n = 6) and on RA patients (n = 6) was determined in flow cytometry analyses after PBMCs isolation by Pancoll separation with anti–HLA-DR, anti-CD2, anti-CD19, anti-CD56, anti-CD14, anti-CD16, and anti-CD115 Ab. Results are shown as mean ± SEM. (C) miR-155 expression on M1-like and M2-like HD macrophages (n = 6) was determined by quantitative real-time PCR. (D) miR-155 mRNA expression in monocytes (HD n = 6, RA n = 6). (E) miR-155 mRNA expression in M1-like macrophages (HD n = 5 and RA n = 6) were determined by qPCR. (F) miR-155 expression in M2-like macrophages (HD n = 5, RA n = 9, RA+ETA n = 7, RA+ADA n = 6, SS n = 7, and SpA n = 7). (G and H) Target mRNA SOCS-1 (G) and C/EBP-β (H) expression on HD (n = 7) and RA (n = 11). Results are show as box and whiskers (min to max). Mann–Whitney t test and Kruskal–Wallis test with Dunn multiple comparisons *p ≤ 0.05.