FIGURE 8.
AntagomiR-155 restored the defect of M2-like polarization. (A) RA Monocytes were transfected with antagomir-155 or control for 18 h and then cultured with human SAB for 6 d. Percentage of CD11HiCD71Hi and geometric mean fluorescence intensity (gMFI) of CD206 and CD163 were determined (n = 6). (B) Spontaneous 6-d cytokine production by M2-like macrophages from RA (n = 6) after AntagomiR-155 or control transfection. (C and D) Representative images and frequencies of CD68+ Arg+ or CD68+ iNOS+ (n = 6). Scale bar, 10 μm. Results are shown as mean ± SEM. Wilcoxon matched-pairs signed rank test. *p ≤ 0.05.