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. 2019 Sep 6;203(7):1830–1844. doi: 10.4049/jimmunol.1900291

FIGURE 9.

FIGURE 9.

Q-PCR analysis of miRNA-150-5p and miRNA-543-3p and specific targeted genes. MDSCs were isolated as described in Fig. 1 legend in vehicle (n = 5) or TCDD (n = 5) mice. (A) Expression of mir-150-5p and mir-543-3p in TCDD-induced MDSCs when compared with vehicle. (B) Expression of targeted genes IL-10, PIM1, ARG2, STAT3, CCL11, CCR3, and CCR5 in TCDD-induced MDSCs when compared with vehicle. (C) Induction of mir-150-5p expression with mimic compared with mock and inhibitor. (D) Induction of IL-10 and PIM1 expression with inhibitor of mir-150-5p compared with mimic of mir-150-5p. (E) Expression of mir-543-3p with mimic compared with mock and inhibitor. (F and G) Expression of ARG2, STAT3, CCL11, CCR3, CCR5, and CXCR2 in the presence of inhibitor of mir-543-3p compared with mimic of mir-543-3p. For (A) and (B), significance was determined using a multiple t test with Holm–Sidak method multiple comparisons test. For (C)–(G), using triplicate (n = 3) wells for each experimental condition, one-way ANOVA with Tukey multiple comparisons test was used to determine significance. Data are representative of at least three independent experiments with reproducible results. Significance was defined as follows: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.