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. 2019 Sep 23;10(10):705. doi: 10.1038/s41419-019-1942-1

Fig. 7. Analysis of miR-29a inhibition of RANKL and CXCL12 expression in bone marrow mesenchymal cells.

Fig. 7

miR-29a compromised the ovariectomy upregulation of RANKL and CXCL12 expression in bone marrow mesenchymal cells (a). The data are expressed mean ± SEM calculated from six mice. Asterisks * and hashtags # indicate significant difference from sham controls and OVX, respectively. Forced miR-29a expression decreased luciferase activity of 3′-UTR of RANKL mRNA (b) and downregulated RANKL mRNA expression and protein abundances, whereas miR-29 interference upregulated RANKL expression (c) of mesenchymal stem cell cultures. miR-29a overexpression reduced PCAF and H3K27ac abundances (d) and decreased H3K27ac enrichment in CXCL12 promoters (e). Forced PCAF expression repressed the miR-29a downregulation of H3K27ac levels and RANKL expression (f). It increased H3K27ac occupancy in CXCL12 promoters and CXCL12 expression (g) in bone marrow mesenchymal cells. Investigations are expressed mean ± SEM calculated from three experiments. Asterisks * and hashtags # indicate significant difference from WT and miR-29aTg/OCN, respectively