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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Chembiochem. 2019 May 27;20(13):1693–1700. doi: 10.1002/cbic.201900037

Figure 2.

Figure 2.

A) Reaction scheme of the mono- and dimethylation of the N2-position by GlaTgs2 and hTgs1. B) RP-HPLC analysis of methylation reactions of 7mGpppAUAU after 2 h incubation (black traces) with GlaTgs and C) after 2.5 h incubation with hTgs1 in comparison with the substrate 7mGpppAUAU (red traces). MALDI-TOF mass spectra of 2m,7mGpppAUAU 5 and 2m,2m,7mGpppAUAU 6 are shown in insets. D) RP-HPLC analysis of N2-methylation reactions of 7mGpppA-(N)17-RNA 2 (18-mer) after 6 h incubation with hTgs1 at 2.5 μM (black trace) and 5 μM (blue trace) in comparison with the N7-methylated substrate 2 (red trace).