Table I.
The information of knockdown, amplification PCR and qPCR primers used in this study.
| Name | Sequence (5′-3′) |
|---|---|
| shMALAT1 S | GATCCCCGGAAGATAGAAACAAGATATTCAAGAGATATCTTGTTTCTATCTTCCTTTTT |
| shMALAT1 AS | AGCTAAAAAGGAAGATAGAAACAAGATATCTCTTGAATATCTTGTTTCTATCTTCCGGG |
| shRNA NC S | GATCCCCTTCTCCGAACGTGTCACGTTTCAAGAGAACGTGACACGTTCGGAGAATTTTT |
| shRNA NC AS | AGCTAAAAATTCTCCGAACGTGTCACGTTCTCTTGAAACGTGACACGTTCGGAGAAGGG |
| CORO1C CDS F | CAAGGATCCATGAGGCGAGTGGTACGACAG |
| CORO1C CDS R | CGCCTCGAGTCAGGCTGCTATCTTTGCCAT |
| MALAT1 amplification PCR F | CACGGATCCGATAAAGGCTGAGTGTTGAG |
| MALAT1 amplification PCR F | CAACTCGAGCTTCGCATACGTGTGTCTGC |
| MALAT1 qPCR F | GACTTCAGGTCTGTCTGTTCT |
| MALAT1 qPCR R | CAACAATCACTACTCCAAGC |
| GAPDH qPCR F | GAAGGTCGGAGTCAACGGAT |
| GAPDH qPCR R | CCTGGAAGATGGTGATGGGAT |
| miR-1-3p RT | GTTGGCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGCCAACATACAT |
| miR-1-3p qPCR F | GCGGGCTGGAATGTAAAG |
| U6 RT | GTTGGCTCTG GTGCAGGGTCCGAGGTATTCGCACCAGAGCCAACAAAATATGG |
| U6 qPCR F | GCTTCGGCAGCACATATACT |
| qPCR Reverse | GTGCAGGGTCCGAGGTATTC |
MALAT1, metastasis-associated lung adenocarcinoma transcript 1; RT, reverse transcription; S, sense; AS, NC, negative control; antisense; F, forward; R, reverse; qPCR, quantitative polymerase chain reaction; miR, microRNA; sh, short hairpin; the coding sequence.