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. 2019 Sep 2;20(4):3565–3572. doi: 10.3892/mmr.2019.10631

Table I.

Sequences of primers.

Primer name Sequence (5′-3′)
TLR1-Forward GGAGGCAATGCTGCTGTT
TLR1-Reverse GCCCAATATGCCTTTGTTATCCTG
TLR2-Forward TGTTGCAAGCAGGATCCAAAG
TLR2-Reverse CACAAAGTATGTGGCATTGTCCAG
TLR3-Forward GGACTTTGAGGCGGGTGTT
TLR3-Reverse TGTTGAACTGCATGATGTACCTTGA
TLR4-Forward AGGATGATGCCAGCATGATGTC
TLR4-Reverse TCAGGTCCAGGTTCTTGGTTGAG
TLR5-Forward AAGATGTCGGAGCCTCAGATG
TLR5-Reverse GGGTCCCTGGTTGTTTAAAGACTTC
TLR6-Forward CAGAGTGAGTGGTGCCATTACGA
TLR6-Reverse AGCCTTCAGCTTGTGGTACTTGTTG
TLR7-Forward TCTTCAACCAGACCTCTACATTCCA
TLR7-Reverse GGAACATCCAGAGTGACATCACAG
TLR8-Forward GCGCTGCTGCAAGTTACGGA
TLR8-Reverse TCGACGATTGCTGCACTCTG
TLR9-Forward GGGACCTCGAGTGTGAAGCA
TLR9-Reverse CTGGAGCTCACAGGGTAGGAA
IL-6-Forward AACCTGAACCTTCCAAAGATGG
IL-6-Reverse TCTGGCTTGTTCCTCACTACT
IL-10-Forward CCTGCCTAACATGCTTCGAG
IL-10-Reverse GAGTTCACATGCGCCTTGAT
TNF-α-Forward ATGAGCACTGAAAGCATGATCC
TNF-α-Reverse GAGGGCTGATTAGAGAGAGGTC
β-actin-Forward GTGGACATCCGCAAAGAC
β-actin-Reverse AAAGGGTGTAACGCAACTAA

IL, interleukin; TLR, toll-like receptor; TNF-α, tumor necrosis factor-α.