Table I.
Primer name | Sequence (5′-3′) |
---|---|
TLR1-Forward | GGAGGCAATGCTGCTGTT |
TLR1-Reverse | GCCCAATATGCCTTTGTTATCCTG |
TLR2-Forward | TGTTGCAAGCAGGATCCAAAG |
TLR2-Reverse | CACAAAGTATGTGGCATTGTCCAG |
TLR3-Forward | GGACTTTGAGGCGGGTGTT |
TLR3-Reverse | TGTTGAACTGCATGATGTACCTTGA |
TLR4-Forward | AGGATGATGCCAGCATGATGTC |
TLR4-Reverse | TCAGGTCCAGGTTCTTGGTTGAG |
TLR5-Forward | AAGATGTCGGAGCCTCAGATG |
TLR5-Reverse | GGGTCCCTGGTTGTTTAAAGACTTC |
TLR6-Forward | CAGAGTGAGTGGTGCCATTACGA |
TLR6-Reverse | AGCCTTCAGCTTGTGGTACTTGTTG |
TLR7-Forward | TCTTCAACCAGACCTCTACATTCCA |
TLR7-Reverse | GGAACATCCAGAGTGACATCACAG |
TLR8-Forward | GCGCTGCTGCAAGTTACGGA |
TLR8-Reverse | TCGACGATTGCTGCACTCTG |
TLR9-Forward | GGGACCTCGAGTGTGAAGCA |
TLR9-Reverse | CTGGAGCTCACAGGGTAGGAA |
IL-6-Forward | AACCTGAACCTTCCAAAGATGG |
IL-6-Reverse | TCTGGCTTGTTCCTCACTACT |
IL-10-Forward | CCTGCCTAACATGCTTCGAG |
IL-10-Reverse | GAGTTCACATGCGCCTTGAT |
TNF-α-Forward | ATGAGCACTGAAAGCATGATCC |
TNF-α-Reverse | GAGGGCTGATTAGAGAGAGGTC |
β-actin-Forward | GTGGACATCCGCAAAGAC |
β-actin-Reverse | AAAGGGTGTAACGCAACTAA |
IL, interleukin; TLR, toll-like receptor; TNF-α, tumor necrosis factor-α.