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. 2019 Aug 29;20(4):3661–3670. doi: 10.3892/mmr.2019.10624

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Copyright: © Cao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Functional role of CENPO in regulating GC cell growth and apoptosis in vitro. CENPO (A) protein and (B) mRNA expression were decreased following transfection with CENPO siRNA. (C) Celigo and (D) MTT assays were performed to determine the proliferation of GC cells transduced with the CENPO siRNA lentivirus. (E) A colony formation assay was performed using GC cells transduced with the CENPO siRNA lentivirus. (F) Number of AGS and MGC-803 cell colonies that formed following transduction with the CENPO siRNA lentivirus. The percentage of apoptotic (G) AGS and (H) MGC-803 cells was significantly increased in the CENPO siRNA group P<0.0001. *P<0.05. CENPO, centromere protein O; GC, gastric cancer; siRNA, small-interfering RNA.