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. 2019 Aug 6;20(4):3276–3284. doi: 10.3892/mmr.2019.10555

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — RNAi-PIT inhibits the Wnt/β-catenin pathway. (A) Reverse transcription-quantitative PCR analysis revealed significant changes in Wif1, β-catenin and sFRP4 mRNA levels in GH3 cells. (B) Western blot analysis revealed a significant change in Wif1, β-catenin and sFRP4 proteins in the Sh-B and Sh-C groups compared with the control group. The protein levels of Wif1 in the Sh-B and Sh-C groups were 4.8±0.52 and 4.2±0.42-fold greater, respectively, than those in the control GH3 cells, while the protein levels of sFRP4 in the Sh-B and Sh-C groups were 3.8±0.43 and 3.5±0.37-fold greater, respectively, than those in the control GH3 cells. n=3. *P<0.05, **P<0.01 vs. respective control. RNAi, RNA interference; SLC20A1, solute carrier family 20 member 1; Wif1, Wnt inhibitory factor 1; sFRP4, secreted frizzled-related protein 4; sh, short hairpin.