Table I.
Primers used in reverse transcription-quantitative PCR.
| Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|
| SLC20A1 | GCTTTATGGTGGTGTTGGCA | GTGTTGTGCTGATGGGAAGG |
| E-CAD | ACTTTGGTGTGGGTCAGGAA | CACATGCTCAGCGTCTTCTC |
| N-CAD | AGAACAGGGTGGACGTCATT | ACCACTGTGACTAGCCCATC |
| MMP-2 | TGCAACCACAACCAACTACG | TAGAGCTCCTGGATCCCCTT |
| MMP-9 | TGGGCAAGCAGTACTCTACC | GTCTTCATGCAGAGGGGAGT |
| Snail | CGAGCAGAGTTGTCTACCGA | CTGCTGGAAGGTGAACTCCA |
| Vimentin | ACTAATGAGTCCCTGGAGCG | AGGTGGCGATCTCAATGTCA |
| VEGF | CACCAAAGCCAGCACATAGG | TTTAACTCAAGCTGCCTCGC |
| GAPDH | AGTCTACTGGCGTCTTCACC | CCACGATGCCAAAGTTGTCA |
SLC20A1, solute carrier family 20 member 1; E-CAD, E-cadherin; N-CAD, N-cadherin; MMP, matrix metalloproteinase; VEGF, vascular endothelial growth factor.