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. 2019 Aug 6;20(4):3317–3325. doi: 10.3892/mmr.2019.10562

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Effect of afatinib on the proliferation, migration and invasion of HCC cells. (A) Cell Cycle Kit-8 assay was performed to investigate cell viability. Relative quantification of (B) migration and (C) invasion of HCC cells. Transwell (D) migration and (E) invasion assay for the migration and invasion ability of HCC cells. n=3. *P<0.05. EGFR, epidermal growth factor receptor; si-CTR, scrambled siRNA; si-EGFR, siRNA targeting EGFR; siRNA, small interference RNA; EGFR-TKI, afatinib treatment; TKI, tyrosine-kinase inhibitor; HCC, hepatocellular carcinoma.