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. 2019 Sep 2;20(4):3849–3857. doi: 10.3892/mmr.2019.10634

Figure 3.

Figure 3.

RMRP interacts with miR-613 in cardiac fibroblasts. (A) An miRcode algorithm was used to predict RMRP-miRNA interaction. RMRP contains one seed sequence that can combine 6 miRNAs. (B) Effect of RMRP siRNA on the miR-613 and miR-1 expression in Ang-II-treated cardiac fibroblasts was assessed using reverse transcription-quantitative PCR. (C) Sequence alignment showing the complementarity between RMRP and miR-613. ‘|’ indicates base pairing. (D) Luciferase activity in cardiac fibroblasts co-transfected miR-613 mimic and luciferase reporters containing RMRP. (E) A diagram and the (F) results of MS2 RIP, performed to detect miR-613 endogenously associated with RMRP. Data are presented as the mean ± standard error of the mean. n=3 independent experiments. **P<0.01 vs. Con; ##P<0.01 vs. Ang-II; &&P<0.01 vs. pmirGLO; $$P<0.01 vs. MS2. miRNA, microRNA; siRNA, small interfering RNA; GFP, green fluorescent protein; Con, control; RMRP, mitochondrial RNA processing endoribonuclease; WT, wild-type; MUT, mutant; RIP, radioimmunoprecipitation; Ang II, angiotensin II.