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. 2019 Aug 23;20(4):3617–3624. doi: 10.3892/mmr.2019.10607

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Copyright: © Lv et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — miR-155 decreased the percentage of CD8⁺ T cells via promoting apoptosis in the cell culture medium. Anti-miR-ctrl, anti-miR-155, pre-miR-ctrl and pre-miR-155 were transfected into Treg cells. (A) Flow cytometry images and (B) the percentage of CD8⁺ cells, as determined at 3 days after transfection. Representative fluorescence-activated cell sorting images from a single case are shown. (C) The apoptosis of CD8⁺ T cells was detected using the Annexin V-FITC/PI Apoptosis Detection kit. (D) Cell apoptosis rate was quantified by flow cytometry. Data are representative of three independent experiments. **P<0.01. miR-155, microRNA-155; Treg, regulatory T; ctrl, control.