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. 2019 Aug 20;20(4):3555–3564. doi: 10.3892/mmr.2019.10592

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Effects of Sr-CaS on BMSC proliferation and apoptosis at 7 days after treatment. (A) The proliferative ability of BMSCs treated with 0, 5 or 10% Sr-CaS at 1, 3, and 7 days after treatment, respectively. *P<0.05 vs. Blank. (B) The soft agar colony formation assay was used to analyze the effect of 0, 5 and 10% Sr-CaS on the proliferative ability of BMSCs. (C) The number of cell colonies was counted. (D) BMSCs were treated with 0, 5 or 10% Sr-CaS for 7 days, and their apoptosis rates were determined by flow cytometry performed with Annexin V/PI staining. (E) The percentage of apoptotic cells was calculated. Sr, strontium; BMSCs, bone marrow stem cells; CaS, calcium sulphate; PI, propidium iodide; OD, optical density; NS, not significant.