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. 2019 Sep 2;20(4):3867–3873. doi: 10.3892/mmr.2019.10639

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Copyright: © Mei et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Overexpression of NR024118 alleviates LPS-induced cell apoptosis in ATDC5 cells. (A) Cell viability was detected by CCK-8 assay. (B) Cell apoptosis was detected by flow cytometry. (C) Quantitative analysis of the flow cytometry data. (D) The expression levels of apoptosis-associated factors Bax, Bcl-2 and Bim was detected by western blot analysis. (E) Quantitative analysis the protein expression. Data are presented as the mean ± standard deviation. ***P<0.001 vs. control group. ^#P<0.05 and ^##P<0.01 vs. LPS group. LPS, lipopolysaccharide; NR024118, pCDNA3.1-NR024118; NC, negative control; Bim, Bcl-2-like protein 11; FITC, fluorescein isothiocyanate.