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. 2019 Aug 5;18(4):2393–2400. doi: 10.3892/etm.2019.7849

Figure 2.

Figure 2.

Relative miR-135a-5p expression during osteogenic differentiation. C2C12 cells were treated with 2 nM BMP2 for 24 h to induce osteogenic differentiation. (A) The relative mRNA expression level of OC, osterix and ALP were determined by RT-qPCR. **P<0.01 vs. Control. (B) The relative miR-135a-5p expression level was determined by RT-qPCR in C2C12 cells following treatment with BMP2 for 0, 6, 12 and 24 h. Data are presented as the mean ± standard deviation from three independent experiments. *P<0.05 and **P<0.01 vs. 0 h. miR, microRNA; BMP2, bone morphogenetic protein 2; OC, osteocalcin; ALP, alkaline phosphatase; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.