Figure 3.

Number of Treg cells following treatment with or without rhIL-6. Number of all Treg cells after culture for 3, 5, 7, 10 and 14 days in the presence of rhIL-2 (300 U/ml) and anti-CD3/CD28 beads (Treg cells/beads, 1:1) (n=8), without or with 25 ng/ml rhIL-6. Unpaired Student's t-test was used to compare differences between groups without and with rhIL-6. Values are expressed as the mean ± standard error of the mean (n=8). *P<0.05, Treg cells cultured for 7 days without vs. with rhIL-6 and **P<0.01, Treg cells cultured for 14 days without vs. with rhIL-6. rhIL, recombinant human interleukin; Treg cells, T-regulatory cells.