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. 2019 Aug 9;18(4):2574–2582. doi: 10.3892/etm.2019.7874

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Copyright: © Zou et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Apoptosis and autophagy-related gene expression in H9c2 cells regulated by MSC exosomes. Immunofluorescence analysis of (A) Apaf1 and (B) ATG13 protein abundances in rat H9c2 cells after H/R and exosome treatment. Cell nuclei were stained using DAPI (magnification, ×120). Reverse transcription-quantitative PCR analysis of (C) Apaf1 and (D) ATG13 gene expression in rat H9c2 cells after H/R and exosome treatment. (E) Western blot analysis of apoptosis and autophagy-related protein expression in H9c2 cells treated with exosomes. GAPDH was used as the internal standard. **P<0.01; #P<0.05, ##P<0.01. MSC, mesenchymal stem cell; Apaf1, apoptotic protease activating factor-1; ATG13, autophagy-related protein 13; H/R, hypoxia-reoxygenation; Exo, exosomes; ns, not significant.