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. 2019 Aug 13;18(4):3002–3008. doi: 10.3892/etm.2019.7880

Figure 4.

Figure 4.

Effects of TUG1 knockdown and overexpression on cell viability. (A) Relative TUG1 expression in normal human bone cell line hFOB and osteosarcoma cell lines MG-63 and U2OS. *P<0.05 vs. hFOB. (B) Relative TUG1 expression in MG-63 and U2OS cells following transfection with plasmid expressing TUG1. *P<0.05 vs. C and NC. (C) Relative TUG1 expression in MG-63 and U2OS cells following transfection with NC or TUG1 siRNA. *P<0.05 vs. C and NC. (D) Effects of TUG1 overexpression on MG-63 and U2OS cell viability. (E) Effects of TUG1 knockdown on MG-63 and U2OS cell viability. *P<0.05 vs. C and NC. Each experiment was repeated three times. For cell viability experiments, all data were normalized to the control group at 96 h. TUG1, taurine upregulated gene 1; siRNA, small interfering RNA; Over, cells transfected with plasmid expressing TUG1; C, controls cells without any transfection; NC, negative control cells transfected with empty vector or negative control siRNA.