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. 2019 Aug 8;18(4):2491–2496. doi: 10.3892/etm.2019.7866

Figure 5.

Figure 5.

SCF signaling promotes P815 cells to secrete IL-13 via the MEK-ERK signaling pathway. P815 cells (cell density, 5×105 cells/ml) were seeded in 24-well plates. After pretreatment for 30 min with U0126 (10 µM), JSI-124 (100 nM) or Wortmannin (1 µM), P815 cells were stimulated for 6 h with SCF (50 ng/ml), then the content of IL-13 in the culture supernatant of P815 cells was detected by ELISA. ‘Control’ means P815 cells stimulated with dissolution medium. ‘SCF’ refers to P815 cells stimulated with 50 ng/ml SCF. ‘JSI-124/U0126/Wortmannin’+SCF’ means P815 cells pretreated for 30 min with JSI-124, U0126 or Wortmannin, followed by stimulation with 50 ng/ml SCF. *P<0.01 vs. control; #P<0.01 vs. SCF. IL-13, interleukin 13; SCF, stem cell factor.