Figure 6.

Effect of SCF signaling on the phosphorylation of ERK in P815 cells. P815 cells (cell density, 5×10⁵ cells/ml) were seeded in 24 well plates. P815 cells were stimulated 0, 15, 30 and 60 min with SCF (50 ng/ml). Subsequently, cytoplasmic proteins were extracted. The phosphorylation of ERK1/2 in cytoplasmic proteins of P815 cells was detected by western blotting. (A) Statistical graph based on densitometric analysis and (B) representative blot. **P<0.01 vs. 0 min. p-, phosphorylated. SCF, stem cell factor.