Effect of the miR-98-5p mimic on MC3T3-E1 cell osteogenic differentiation. Osteoblast phenotypes were assessed by determining the expression of osteoblast marker genes and extracellular ALP activity. (A) Extracellular ALP activity in MC3T3-E1 cells. (B) Protein level of ALP, Osterix and Runx2 and mRNA levels of (C) ALP, (D) Osterix and (E) Runx2. Data are presented as the mean ± standard deviation. **P<0.01 vs. Control; ##P<0.01 vs. Induction; &&P<0.01 vs. mimic. miR, microRNA; ALP, alkaline phosphatase; Osterix, transcription factor sp7; Runx2, runt related transcription factor 2.