Table I.
Primer sequence (5′-3′) | ||
---|---|---|
Primer name | Forward | Reverse |
H-miR-192 | RT primer: CUGACCUAUGAAUUGACAGCCGTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACGGCTGTCA | |
GGGCTGACCTATGAATTG | CAGTGCGTGTCGTGGAGT | |
H-U6 | RT primer: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAAAAT | |
ACGATGCACCTGTACGATCA | TCTTTCAACACGCAGGACAG | |
H-TGF-β1 | GGACCAGTGGGGAACACTAC | AGAGTCCCTGCATCTCAGAGT |
H-Smad3 | GTGCTGGGGTTAGGTCACTG | GAATGTCGCATCCTGTGGGA |
H-Smad7 | GGAGGTCATGTTCGCTCCTT | GTTTGGTCCTGAACATGCGG |
H-α-SMA | GAGGGAAGGTCCTAACAGCC | TAGTCCCGGGGATAGGCAAA |
H-Col1 | GCTCGTGGAAATGATGGTGC | ACCCTGGGGACCTTCAGAG |
H-GAPDH | GAAAGCCTGCCGGTGACTAA | AGGAAAAGCATCACCCGGAG |
R-miR-192 | RT: CUGCCAGUUCCAUAGGUCACAGGTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACCTGTGACC | |
GCGCTGCCAGTTCCATAGG | CAGTGCGTGTCGTGGAGT | |
R-U6 | RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAAAAT | |
ACGATGCACCTGTACGATCA | TCTTTCAACACGCAGGACAG | |
R-TGF-β1 | GACTCTCCACCTGCAAGACC | GGACTGGCGAGCCTTAGTTT |
R-Smad3 | CTGGGCAAGTTCTCCAGAGTT | AAGGGCAGGATGGACGACAT |
R-Smad7 | GAGTCTCGGAGGAAGAGGCT | CTGCTCGCATAAGCTGCTGG |
R-α-SMA | CATCACCAACTGGGACGACA | TCCGTTAGCAAGGTCGGATG |
R-Col1 | GTACATCAGCCCAAACCCCA | CAGGATCGGAACCTTCGCTT |
R-GAPDH | AGTGCCAGCCTCGTCTCATA | GGTAACCAGGCGTCCGATAC |
H, Human; R, Rat; miR, microRNA; TGF-β1, transforming growth factor-β1; α-SMA, α-smooth muscle actin; col1, collagen type 1; RT, reverse transcription.