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. 2019 Aug 14;18(4):3053–3061. doi: 10.3892/etm.2019.7887

Table I.

Primer sequences.

Primer sequence (5′-3′)

Primer name Forward Reverse
H-miR-192 RT primer: CUGACCUAUGAAUUGACAGCCGTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACGGCTGTCA
GGGCTGACCTATGAATTG CAGTGCGTGTCGTGGAGT
H-U6 RT primer: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAAAAT
ACGATGCACCTGTACGATCA TCTTTCAACACGCAGGACAG
H-TGF-β1 GGACCAGTGGGGAACACTAC AGAGTCCCTGCATCTCAGAGT
H-Smad3 GTGCTGGGGTTAGGTCACTG GAATGTCGCATCCTGTGGGA
H-Smad7 GGAGGTCATGTTCGCTCCTT GTTTGGTCCTGAACATGCGG
H-α-SMA GAGGGAAGGTCCTAACAGCC TAGTCCCGGGGATAGGCAAA
H-Col1 GCTCGTGGAAATGATGGTGC ACCCTGGGGACCTTCAGAG
H-GAPDH GAAAGCCTGCCGGTGACTAA AGGAAAAGCATCACCCGGAG
R-miR-192 RT: CUGCCAGUUCCAUAGGUCACAGGTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACCTGTGACC
GCGCTGCCAGTTCCATAGG CAGTGCGTGTCGTGGAGT
R-U6 RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAAAAT
ACGATGCACCTGTACGATCA TCTTTCAACACGCAGGACAG
R-TGF-β1 GACTCTCCACCTGCAAGACC GGACTGGCGAGCCTTAGTTT
R-Smad3 CTGGGCAAGTTCTCCAGAGTT AAGGGCAGGATGGACGACAT
R-Smad7 GAGTCTCGGAGGAAGAGGCT CTGCTCGCATAAGCTGCTGG
R-α-SMA CATCACCAACTGGGACGACA TCCGTTAGCAAGGTCGGATG
R-Col1 GTACATCAGCCCAAACCCCA CAGGATCGGAACCTTCGCTT
R-GAPDH AGTGCCAGCCTCGTCTCATA GGTAACCAGGCGTCCGATAC

H, Human; R, Rat; miR, microRNA; TGF-β1, transforming growth factor-β1; α-SMA, α-smooth muscle actin; col1, collagen type 1; RT, reverse transcription.