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. 2019 Aug 5;18(4):2592–2598. doi: 10.3892/etm.2019.7854

Figure 2.

Figure 2.

Effect of 3F plasmid in combination with Pax4 overexpression on the expression of genes associated with the differentiation of human umbilical cord mesenchymal stem cells to pancreatic β-like cells. The genes assessed included NKX6-1, SLC2A2, GCK, PCSK1, NEUROD1, ISL1, PAX6 and PCSK2. Values are expressed as the mean ± standard deviation (n=5). *P<0.05, **P<0.01, ***P<0.001 vs. EGFP group; #P<0.05, ##P<0.01 vs. 3F group. Groups: EGFP, cells transfected with EGFP plasmid; 3F, cells transfected with Pdx1/MafA/Ngn3 plasmid; Pax4, cells transfected with Pax4 plasmid; 3F/Pax4, cells transfected with Pdx1/MafA/Ngn3 plasmid and Pax4 plasmid. Pdx1, pancreatic and duodenal homeobox 1; MafA, MAF bZIP transcription factor A; Ngn3, neurogenin 3; Pax4, paired box 4; EGFP, enhanced green fluorescence plasmid; GCK, glucokinase; NKx6.1, NK6 homeobox 1; SLC2A2, glucokinase; PCSK1, proprotein convertase subtilisin/kexin type 1; NEUROD1, neuronal differentiation 1; ISL1, ISL LIM homeobox 1; PCSK2, proprotein convertase subtilisin/kexin type 2.