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. 2019 Aug 16;18(4):2901–2908. doi: 10.3892/etm.2019.7909

Figure 4.

Figure 4.

Effects of A2 knockdown or overexpression combined with the autophagy inhibitor 3-MA treatment on OGD-induced injury in human retinal endothelial cells. (A and B) Cells were infected with a lentiviral vector encoding shRNAs against human A2 or a lentiviral scramble control vector (vector 1) for 48 h, then treated with OGD for another 24 h. (A) Cell viability and (B) expression of cleaved-caspase 3 were measured. (C and D) Cells were infected with a lentiviral A2 overexpression vector or a lentiviral control vector (vector 2) for 48 h, then treated with OGD for another 24 h in the presence or absence of 3-MA (5 mM). (C) Cell viability and (D) expression of cleaved-caspase 3 were measured. **P<0.01 vs. Con group; #P<0.05 vs. OGD group, &P<0.05 vs. OE-A2+OGD group. 3-MA, 3-methyladenine; A2, Annexin A2; OE-A2, lentiviral A2 overexpression vectors; sh-A2, lentiviral vectors encoding short hairpin RNAs against human A2; OGD, oxygen-glucose deprivation; Con, control.