Figure 2.

Intracellular Mg²⁺ concentration and Ca²⁺ influx in endothelium. A–C) siRNA knockdown of TRPM7, MagT1, and SLC41A1. Western blots showing expression of specific proteins in endothelial cells after treated with control siRNA or siRNA against TRPM7, MagT1, or SLC41A1; and quantitative measurements of relative protein levels from Western blots. (*P < 0.05, compared to control group; n = 4). D) Cells were transfected with siRNA against TRPM7, MagT1, SLC41A1, or scramble control siRNA, and were incubated with Mg²⁺‐free Ca²⁺‐containing medium for 15 min before being treated with high Mg²⁺ (2 mmol L⁻¹ “+”) or Mg²⁺‐deficiency (0.1 mmol L⁻¹ “–”). Mag fura‐2AM intensity was measured one minute after Mg²⁺ addition. (*P < 0.05, vs Mg²⁺‐deficiency or group treated with MagT1 siRNA and TRPM7 siRNA; **P < 0.01, vs Mg²⁺‐deficiency or group treated with MagT1 siRNA and TRPM7 siRNA; n = 3). E) Mg²⁺ inhibits Ca²⁺ influx in endothelium as an antagonist of Ca²⁺. Intracellular Ca²⁺ concentration measured by fura‐2AM intensity. Fura‐2AM intensity were recorded before ionomycin addition as basal level and one minute after ionomycin (10⁻⁶ mol L⁻¹) addition. (* P < 0.05, vs Mg²⁺‐deficiency; n = 4).