Figure 7.

Mg²⁺ regulates endothelial barrier‐stabilizing mediators. A) Relative intracellular cAMP level as compared to Mg²⁺‐deficiency (0.1 mmol L⁻¹) measured 1 h after Mg²⁺ (2 mmol L⁻¹) addition. B) Relative intracellular PKA activity as compared to Mg²⁺‐deficiency (0.1 mmol L⁻¹) measured 1 h after Mg²⁺ (2 mmol L⁻¹) addition. C) Relative mRNA level of key endothelial barrier permeability mediator FGF1, FGF2, VEGFA, and eNOS as compared to Mg²⁺‐deficiency (0.1 mmol L⁻¹) measured 24 h after Mg²⁺ (2 mmol L⁻¹) addition. D) Relative protein expression level of key endothelial barrier permeability mediator FGF1, FGF2, VEGFA, and eNOS as compared to Mg²⁺‐deficiency (0.1 mmol L⁻¹) measured 24 h after Mg²⁺ (2 mmol L⁻¹) addition. E) Phosphorylation of Akt with or without Mg²⁺ treatment. (* P < 0.05, vs Mg²⁺‐deficiency or group treated with MagT1 siRNA and TRPM7 siRNA; ** P < 0.01, vs Mg²⁺‐deficiency or group treated with MagT1 siRNA and TRPM7 siRNA; n = 3).