Figure 9.

Mg²⁺‐deficiency enhances endothelial barrier permeability in vivo. A) Quantitation of Evans Blue extravasation in different tissues of Mg²⁺‐deficiency mouse and control mouse (Mg²⁺‐treatment). B) Quantitation of Evans Blue extravasation in liver. C) Quantitation of Evans Blue extravasation in lung. D) Quantitation of Evans Blue extravasation in kidney. E) Quantitation of Evans Blue extravasation in skin. F) Quantitation of Evans Blue extravasation in brain. Normal mouse was treated with histamine to induce transient vessel hyper‐permeability, and may also be subjected to Mg²⁺‐treatment with or without S1P1 antagonist W146. (* P < 0.05, vs Mg²⁺‐deficiency; ** P < 0.01, vs Mg²⁺‐deficiency; *** P < 0.001, vs Mg²⁺‐deficiency; n = 6).