Figure 1.

Proteins and protein materials. A) Schematic representation of the fusion proteins FN‐p31‐H6 and Omo‐FN‐H6, indicating the molecular mass of the products. Control proteins used in the study (FN‐GFP‐H6 and GFP‐H6,26) are also included. Box sizes are only approximate. B) Coomassie blue staining of a SDS‐PAGE gel loaded with purified proteins. Numbers on the left indicate the molecular masses in kDa of the ladder marker. On the right, arrows indicate the position of the full‐length recombinant proteins. M indicates the molecular marker line, and p31 and O indicate FN‐p31‐H6 and Omo‐FN‐H6 lanes, respectively. C) Internalization of FN‐GFP‐H6 in cultured CD44⁺ MDA‐MB‐231 and CD44⁻ HepG2 cells, measured as the % of green fluorescent cells. Protein was added at 0.5 × 10⁻⁶ m and exposed to cells for 24 h. The percentage of CD44⁺ cells in each cell line is also indicated as a reference. D) Representative FESEM images of FN‐p31‐H6 and Omo‐FN‐H6 IBs at two different magnifications (zoom in the insets). Magnifications are equivalent in each micrograph pair to allow comparative visualization. Magnification bars represent 500 nm.