Figure 3.

Selective accumulation of AON in the inflamed colons of mice with acute colitis. A) Representative ex vivo images (left) and quantitative analysis (right) illustrating Cy7.5‐AON distribution in the colons of mice with or without DSS‐induced acute colitis at 4 h after oral administration. The fluorescent signals of Cy7.5 revealed the accumulation of Cy7.5‐AON in the colons. B) Typical fluorescence images (left) and quantitative analysis (right) of the colonic tissues from colitis mice treated with free Cy7.5‐Ac2‐26 or Cy7.5‐AON. C) The area under the fluorescence intensity–time curve (AUC) of Cy7.5 fluorescence in colonic tissues after treatment with Cy7.5‐Ac2‐26 or Cy7.5‐AON. D) Fluorescence images (left) and quantitative analysis (right) of blood samples from colitis mice treated with free Cy7.5‐Ac2‐26 (top panel) or Cy7.5‐AON (bottom panel). E) The AUC value of Cy7.5 fluorescence of blood samples after treatment with Cy7.5‐Ac2‐26 or Cy7.5‐AON. F) Fluorescence images of cryosections of colonic tissues from mice with DSS‐induced acute colitis at 4 h after treatment with FITC‐Ac2‐26 or FITC‐AON. The green fluorescence indicates the presence of FITC‐labeled Ac2‐26, while the blue fluorescence reveals DAPI‐labeled nuclei. Scale bars, 100 µm. Representative flow cytometry plots (left) and quantitative analysis (right) indicating G) the percentage of Cy5‐AON‐containing cells and H) total fluorescence intensity of cells with Cy5‐AON. In both cases, cells were isolated from the healthy or inflamed colon tissues. The colitis group represents cells from diseased mice without treatment with Cy5‐AON. Distribution of Cy5‐AON in I) neutrophils and J) macrophages. The left panels show representative flow cytometry contour plots, while the right panels indicate corresponding quantitative data. All quantitative data are presented as mean ± SE (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001; ns, no significance.