Fig. 6.

rSj16 protein and Sj16 peptide increased M2 macrophages in liver of S. japonicum-infected mice. a Sections taken from livers of S. japonicum-infected mice treated with PBS, GST protein (50 µg/d), rSj16 (50 µg/d), Sj16 peptide (50 µg/d) for 7 consecutive days before the animals sacrificed at 8 weeks post-infection. Co-immunostaining of F4/80 and Arg-1, photographed at 40×. b Liver leukocytes were harvested from 10 week S. japonicum-infected mice administered PZQ alone (150 mg/kg/day for 3 days), Sj16 peptide alone (50 µg/d for 5 weeks), or combined (PZQ: 150 mg/kg/day for 3 days; Sj16 peptide: 50 µg/d for 5 weeks) at week 5 week post-infection. The expression of F4/80⁺ CD206⁺ (M2) macrophages in the liver were evaluated by FCM analysis. c The expression of IL-10 and TGF-β in the liver was identified by immunohistochemistry using IL-10 and TGF-β antibody, respectively. Arrows indicate the positive signal. Results are expressed as the mean ± SEM of 2 independent experiments (n = 6). Significant differences were detected, *P < 0.05