Table 1.
Bacterial HBGA and lectin binding activity with corresponding Western blot band size
| HBGA/Lectin | H/Ulex europaeus (α-l-fucose: H type 2) | AB/Dolichos biflorus (GalNAc: A type 1) | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Protein size (kDa) | 140 | 100 | 70 | 50 | 35 | 25 | 17 | 15 | 140 | 100 | 70 | 50 | 35 | 25 | 17 | 15 |
| S. aureus | −/+ | +/− | +/− | +/+ | +/− | +/− | +/− | |||||||||
| E. cloacae | +/+ | +/− | −/+ | +/+ | −/+ | |||||||||||
| Klebsiella spp. | +/+ | −/+ | +/− | +/+ | −/+ | |||||||||||
| Bacillus spp. | −/+ | −/+ | +/− | +/+ | +/+ | +/+ | +/− | +/+ | ||||||||
| E. faecium | −/+ | +/+ | +/− | −/+ | ||||||||||||
| Citrobacter spp. | +/+ | +/+ | −/+ | +/− | +/− | +/+ | −/+ | |||||||||
| H. alvei | +/− | +/+ | −/+ | +/− | +/− | +/+ | ||||||||||
| HBGA/Lectin | B/Bandeiraea simplicifolia (α -d-Gal: B) | |||||||
|---|---|---|---|---|---|---|---|---|
| Protein size (kDa) | 140 | 100 | 70 | 50 | 35 | 25 | 17 | 15 |
| S. aureus | +/+ | +/− | +/− | |||||
| E. cloacae | +/− | +/− | +/− | −/+ | ||||
| Klebsiella spp. | +/− | +/− | −/+ | |||||
| Bacillus spp. | +/+ | −/+ | +/− | |||||
| E. faecium | +/+ | |||||||
| Citrobacter spp. | +/− | +/− | +/− | −/+ | +/− | |||
| H. alvei | +/− | +/− | +/+ | +/− | ||||
+ Indicates band observed at corresponding kDa and − indicates no band observed in three replicates. Each HBGA result (left side of each column) is coupled with the specific lectin sugar targeted from that residue (right side of each column)