Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Sep 23;10:284. doi: 10.1186/s13287-019-1363-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 1 — Generation of hiPSC-derived RGCs. a Schematic of the protocol for differentiating hiPSCs into RGCs. Culturing conditions (suspension or adherent) are shown at the top in green, crucial morphogenic factors shown at the top in blue, the intermediate stages EB—embryoid bodies, NR—neural rosettes, and OV—optic vesicles. b Bright-field images of RGCs after dissociation from optic vesicles from day 1 to day 21. c Flow cytometry analysis of the proportion of Thy-1-expressing cells in RGC populations differentiated by original and modified methods. d Western blot analysis demonstrating expression of positive RGC markers in mature day 21 hiPSC-derived RGCs as compared to neural rosette (NR) stage. e Expression of positive RGC markers in the mature day 21 RGCs demonstrated by immunofluorescent staining. f Electrophysiological analysis of the mature day 21 RGCs (bottom) compared to immature day 21 RGCs (top)