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. 2019 Sep 23;10:285. doi: 10.1186/s13287-019-1376-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Schematic description for human umbilical cord preparation to improve the transduction of MSC-EM-initiating cells. Tweezers (4 mm) were used to generate uniform slices by transversal dissection of the umbilical cord with a scalpel. In the second step, the slices were cut between the blood vessels to ensure one blood vessel in each of three pieces (Standard). One blade of the scissors was pushed into the blood vessel lumen and closed in the orientation that the second blade cut through the surface of the umbilical cord and opened the blood vessel (BLOP). In the last step, the blood vessel was removed entirely by simply pulling out the blood vessel (BLRV)