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. 2019 Sep 23;10:285. doi: 10.1186/s13287-019-1376-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Transcriptome and secretome analyses of early and late induced MSC-EMs after activation. Early and late induced MSC-EMs were activated with IFN-γ and TNF-α for 4 days. a Heat map of the transcriptome analysis. b For conditioning, cells were washed with PBS and cultured in MSC10 for 24 h. The media were analyzed with the Bio-Plex Pro™ Human Cytokine 27-plex Assay and the 5 most deregulated cytokines are shown. Cytokines and the concentration range (pg/mL) detected are given in the column headers on the right of the graphs. A paired Wilcoxon signed-rank test was performed and confirmed significantly more produced cytokines by later induced cultures (p < 9.8e−06)