FIG 2.

Loss of fatty acid cyclopropanation or dioctylamine inhibition of H. pylori growth. (A) Growth curves for H. pylori strain 26695, strain 26695 ΔcfaS, strain 26695 ΔcfaS::cfaS, strain 26695 supplemented with 10 μM dioctylamine (Dioc), and strain 26695 supplemented with 50 μM dioctylamine at 37°C in BHI broth containing FCS. (B) Dioctylamine resistance. H. pylori strain 26695 overexpressing CfaS (HpS49) was more resistant to dioctylamine than strain 26695 with vector alone (HpS48). The data are means, with standard error bars, from three independent determinations, analyzed by Student's t test.