FIG 7.

Native PAGE analysis shows evidence of a YrrN-YrrO complex. Increasing concentrations of His₆-YrrN are incubated with 2 μM His₆-YrrO in 30 mM Tris-glycine with 5 mM DTT (20 μl total volume) for 30 min at RT and then analyzed on a 10% nondenaturing polyacrylamide gel using 30 mM Tris-glycine, pH 7.5, as the buffer. Gel was stained with Coomassie blue. Lane 1, 2 μg bovine serum albumin; lane 2, 2 μg purified His₆-YrrN; lane 3, 4 μg YrrN; lane 4, 2 μg purified His₆-YrrO; lane 5, 2 μg YrrO plus 0.125 eq YrrN; lane 6, YrrO plus 0.25 eq YrrN; lane 7, YrrO plus 0.5 eq YrrN; lane 8, YrrO plus 1 eq YrrN; lane 9, YrrO plus 2 eq YrrN. Arrows show position of major (A) and minor (B) potential YrrNO complexes.