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Thermodynamics parameters of peptide binding to HpDppA
| Method | Ligand | Mean value ± SDa |
||
|---|---|---|---|---|
| Kd at 25°C (μM) | Enthalpy (ΔH) (cal/mol) | Entropy (ΔS) (cal/mol/degree) | ||
| Isothermal titration calorimetry | VYIHPF | 7.78 ± 0.65 | 8,474 ± 1,121 | 51.8 ± 3.7 |
| STSA | 5.85 ± 0.56 | −11,800 ± 2,249 | −15.6 ± 7.6 | |
| Thermal shift assay | VYIHPF | 7.15 ± 0.11 | ND | ND |
| STSA | 7.06 ± 0.18 | ND | ND | |
| AH | 9.02 ± 0.01 | ND | ND | |
| AI | 9.04 ± 0.24 | ND | ND | |
| IS | 9.38 ± 0.40 | ND | ND | |
Values shown are from a triplicate experiment. ND, not determined.
